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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has actually been noticed in lots of tumors, which results in chromosome instability, and therefore may perhaps contribute to tumorigenesis. This protein was discovered to associate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle regulated histone gene expression and performs a vital function in advertising and marketing mobile-cycle progression inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already described. Two supplemental splice variants were being noted but in depth nucleotide sequence info just isn't yet out there.
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Blocking peptides are peptides that bind exclusively into the target antibody and block antibody binding. These peptide generally is made up of the epitope regarded from the antibody.
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This tutorial outlines more doable optimization of mobile and immunoassay parameters to ensure the best possible final results are...
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This information outlines even more feasible optimization of mobile and immunoassay parameters to be sure the best possible benefits are...
Antibodies sure to the blocking peptide not bind to your epitope on the target protein. By comparing the staining from your blocked antibody versus the antibody by yourself, one can see which staining is precise.
Goal Information Cyclin E belongs to the hugely conserved cyclin household, whose users are characterized by a spectacular periodicity in protein abundance with the cell cycle. Cyclins operate as regulators of CDK kinases. Distinctive cyclins exhibit distinct expression and degradation patterns which contribute towards the temporal coordination of each and every mitotic occasion. Cyclin E forms a complex with and capabilities to be a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates with the G1-S stage boundary and it is degraded as cells development by S stage.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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